Jarek Krol, Postdoctoral Scientist

Dr. Jarek Król, Postdoctoral Scientist

…Publications

Articles

2008

J. Król, Mazur A., M. Marczak, A. Skorupska 2008, Application of physical and genetic map of Rhizobium leguminosarum bv. trifolii TA1 to comparison of three closely related rhizobial genomes. MGG 279, 2, 107-121.

Olender,A., Król,J.E., Ruminski,W., Zawadzki,A. and Niemcewicz,M. 2008. Corynebacterium diphtheriae (tox-) - participation in two cases of atypical infection. FEMSIM - submitted

2007

J. Król, Mazur A., M. Marczak, A. Skorupska 2007, Syntenic arrangements of the surface polysaccharide biosynthesis genes in Rhizobium leguminosarum. Genomics 2007 89, 2, 237-247.

2006

Skorupska A, Janczarek M, Marczak M, Mazur A, J. Król 2006, Rhizobial exopolysaccharides: genetic control and symbiotic functions. Microb Cell Fact. 16, 5-17.

M. Marczak, Mazur A., J. Król, Gruszecki W.I., A. Skorupska 2006 Lipoprotein PssN of Rhizobium leguminosarum bv. trifolii: subcellular localization and possible involvement in exopolysaccharide export. J. Bacteriol. 188, 6943-6952

Chudnicka A., J. Król, Koziol-Montewka M., Bogut A. 2006 Propionibacterium – a pathogen, an opportunistic microorganism. Polish J. Environ. Stud. 15, 2b, 1038-1041

Skorupski P., J. Król, Starega J., Adamiak A., Jankiewicz K., Rechberger T. 2006. An alpha-1 chain of type I collagen Sp1-binding site polymorphism in women suffering from stress urinary incontinence. Am J Obstet Gynecol.194, 346-350

2005

Mazur A., M. Marczak, J. Król, A. Skorupska 2005. Topological and transcriptional analysis of pssL gene product: a putative Wzx-like exopolysaccharide translocase in Rhizobium leguminosarum bv. trifoliiTA1. Arch. Microbiol. 184, 1-10.

2004

Wielbo J., Mazur A., J. Król, Marczak M., Kutkowska J. and Skorupska A. 2004. Complexity of phenotypes and symbiotic behaviour of Rhizobium leguminosarum biovar trifolii exopolysaccharide mutants. Arch. Microbiol. 182, 331 – 336.

2003

Mazur A., J. Król, M. Marczak, A. Skorupska 2003. Membrane topology of PssT, the transmembrane protein component of the type I exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii strain TA1. J. Bacteriol. 185, 2503-2511.

2002

Mazur A., J. Król, J. Wielbo, T. Urbanik-Sypniewska, A. Skorupska 2002. Rhizobium leguminosarum bv. trifolii PssP protein is required for exopolysaccharide biosynthesis and polymerization. Mol. Plant Microbe Interact. 15, 388-397.

2001

Mazur A., J. Król, A. Skorupska 2001. Rhizobium leguminosarum bv. trifolii pssNOP genes encoding proteins involved in polymerization and translocation of exopolysaccharide. DNA Sequence 12, 1-12.

Janczarek M., J. Król, J. Kutkowska, A. Mazur, J. Wielbo, W. Borucki, J. Kopcinska, B. Lotocka, T. Urbanik-Sypniewska, A. Skorupska 2001. Mutation in the pssB-pssA intergenic region of Rhizobium leguminosarum bv. trifolii affects the surface polysaccharides synthesis and nitrogen fixation ability, J Plant Physiol. 158, 1565-1574.

2000

Skorupska A., Janczarek M., J. Król, Marek-Kozaczuk M., Mazur A., Wielbo J. 2000. Biotic stress during symbiosis in leguminous plants. In: Stress in biological and medical research. Lublin, 2000. str. 9-15.

Lorkiewicz Z., Lisova N., Skorupska A., Russa, R.,Glowacka M., Kowalczuk E., Urbanik-Sypniewska T., Choma A., Malek W., Galan M., J. Król, Kalabun J., Szyprowska S., Marek-Kozaczuk M., Turska A., Wdowiak S., Janczarek M., Dolecka J., Mazur A., Melke A. 2000. The Rhizobium – Plant symbiosis. W: Bull. Polish Academy of Science, Lublin. pp. 51-60.

1999

Janczarek M., Mazur A., Wielbo J., J. Król, Skorupska A. 1999. Rhizobial exopolysaccharides: structures, biosynthesis and function in symbiosis. Postepy Mikrobiologii. 38, 217-244.

Janczarek M., J. Król, A. Skorupska 1999. The pssB gene productsof Rhizobium leguminosarum bv trifolii is homologous to a family of inositol monophosphatase. FEMS Microbiol. Lett. 173, 319-325.

1998

J. Król, J. Wielbo., A. Mazur., J. Kopcinska, B. Lotocka, W. Golinowski, A. Skorupska, 1998. Molecular characterization of pssCDE genes of Rhizobium leguminosarum bv. trifolii strain TA1: pssD mutant is affected in exopolysaccharide synthesis and endocytosis of bacteria. Mol. Plant-Microbe Interact. 11. 1142-1148

Mazur A., J. Wielbo, J. Król, J. Kopcinska, B. Lotocka, W. Golinowski, A. Skorupska, 1998. Molecular characterization and symbiotic importance of prsD gene of Rhizobium leguminosarum bv. trifolii TA1. Acta Bioch. Polon. 45, 1067-1073.

1997

J. Król, A. Skorupska, 1997. Identification of the genes in Rhizobium leguminosarum bv. trifolii whose products are homologues to a family of ATP-binding proteins. Microbiology 143, 1389-1394

1996

J. Król, M. Janczarek, A. Wojcieszuk, A. Skorupska, 1996. The clover (Trifolium sp.) and Rhizobium leguminosarum bv. trifolii symbiosis: a system for study the significance of acidic polysaccharides. Bull. Polish Academy of Science, 44, 1-5.

1995

Skorupska A., J. Król, 1995. Extracellular polysaccharides in Rhizobium: their role in symbiosis with clover plants. Kosmos, 44, (3/4), 589-600.

Skorupska A., J. Król, 1995. Nodulation response of exopolysaccharide deficient mutants of Rhizobium leguminosarum bv. trifolii to addition of acidic polysaccharide. Microbiol. Res. 150; 297-303.

Conference Papers – selected

2006

Król J.E., Mazur A., Marczak M., Bigoraj E, Skorupska A. 2006. Construction of the physical map of Rhizobium leguminosarum bv. trifolii TA1 genome. II Konferencja Komitetu Mikrobiologii PAN, Lublin 2006. Materialy Zjazdowe, str. 53.

Król J.E., Mazur A., Marczak M., Kepka M, Skorupska A. 2006. Identification and in silico analysis of Rhizobium leguminosarum bv. trifolii TA1 polysaccharide biosynthesis regions. II Konferencja Komitetu Mikrobiologii PAN, Lublin 2006. Materialy Zjazdowe, str. 54.

Marczak M., Mazur A., Król J., Skorupska A. 2006. Secondary structure and topolygy of PssN lipoprotein nenber of the exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii TA1. II Konferencja Komitetu Mikrobiologii PAN, Lublin 2006. Materialy Zjazdowe, str. 59.

Mazur A., Król J., Marczak M., Górniak A., Skorupska A. 2006. Identification and in silico analysis of Rhizobium leguminosarum bv. trifolii TA1 polysaccharide biosynthesis plasmid region. II Konferencja Komitetu Mikrobiologii PAN, Lublin 2006. Materialy Zjazdowe, str. 60.

Chudnicka A., Król J., Walory J., Koziol-Montewka M. 2006. Characteristic of nontoxic strain of Corynebacterium dipphteriae isolated from blood. II Konferencja Komitetu Mikrobiologii PAN, Lublin 2006. Materialy Zjazdowe, str. 39.

Turska-Szewczuk A., Kutkowska J., Król J., Urbanik-Sypniewska T., Dolecka J., Pietras H., Koltun J., Russa R. 2006. Altered distribution of lipopolysaccharide O-antigenic chain of Mesorhizobium loti NZP2213.1 prevents the establishment of effective symbiosis with Lotus corniculatus. II Konferencja Komitetu Mikrobiologii PAN, Lublin 2006. Materialy Zjazdowe, str. 60.

Król J.E., Mazur A., Marczak M. and Skorupska A. 2006. Physical and genetic map of the Rhizobium leguminosarum bv. trifolii TA1 genome. 7th European Nitrogen Fixation Conference, Aarhus (Denmark). p. 50.

Marczak M., Mazur A., Król J., Gruszecki W., Skorupska A. 2006. Secondary structure and topology of PssN lipoprotein, a component of exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii TA1. 7th European Nitrogen Fixation Conference, Aarhus (Denmark). p. 51.

Mazur A., Król J., Marczak M., Skorupska A. 2006. In silico analysis of polysaccharide biosynthesis clusters in Rhizobium leguminosarum bv. trifolii TA1. 7th European Nitrogen Fixation Conference, Aarhus (Denmark). p. 52.

Król J.E., Mazur A., Marczak M. and Skorupska A. 2006. A genomic approach to analyse polysaccharide biosynthesis genes in Rhizobium leguminosarum bv. trifolii. 7th European Nitrogen Fixation Conference, Satellite meeting on Genomics of Nitrogen-Fixing Organisms, Aarhus (Denmark). p. 178.

2005

Król J.E., Mazur A., Marczak M. and Skorupska A. 2005. Mapping of the Rhizobium leguminosarum bv. trifolii TA1 genome. The First Baltic Sea Region Symposium and Postgraduate course "Agro-biotechnology focused on root-microbe systems", Kowno (Litwa). Materialy Zjazdowe, str. 33-34.

Król J.E., Mazur A., Marczak M. and Skorupska A. (2005) Mapping of the Rhizobium leguminosarum bv. trifolii TA1 genome. 40th Meeting of The Polish Biochemical Society, Lublin 2005. Materialy Zjazdowe, str. 9.

2004

Mazur A., Marczak M., Król J., Skorupska A. 2004. Topological and functional analysis of PssL protein, putative exopolysaccharide transporter in Rhizobium leguminosarum bv. trifolii TA1. Proceedings of the Sixth European Nitrogen Fixation Conference, Toulouse (Francja), nr sekcji P3.15, str. 40.

Król J., Mazur A., Marczak M., Rybak A., Komisarczuk A., Skorupska A. 2004. Identification of exopolysaccharide biosynthesis region in Rhizobium leguminosarum bv. trifolii BAC library. Proceedings of the Sixth European Nitrogen Fixation Conference, Toulouse (Francja), nr sekcji P3.23, str. 44.

2003

Mazur A., Marczak M., Król J., Skorupska A. 2003. Using the gene fusions in topology studies of Rhizobium leguminosarum bv. trifolii exopolysaccharide membrane transporters. Materialy II Krajowego Kongresu Biotechnologii, Lódz, str. 230.

2002

Schulmeister S. A., Król J., Vorhoelter F., Skorupska A., Lotz W. 2002. Sequence analysis of the Sinorhizobium meliloti 2011 temperate bacteriophage PBC5. Fifth European Nitrogen Fixation Conference, Norwich, UK.

Mazur A., Król J., Marczak M., Skorupska A. 2002. Membrane topology of the Rhizobium leguminosarum bv. trifolii TA1 PssT protein. Fifth European Nitrogen Fixation Conference, Norwich, UK. No.3.10

Król J., Mazur A., Olech B., Skorupska A. 2002. Identification and analysis of the pssT gene of Rhizobium leguminosarum bv. trifolii TA1. Fifth European Nitrogen Fixation Conference, Norwich, UK. No. 4.5

2001

Mazur A., Król J. Skorupska A. 2001. Function of the PssP protein in exopolysaccharide biosynthesis in Rhizobium leguminosarum bv. trifolii TA1 Materialy XIV Zjazdu P.T. Genet., Poznan, str. 88.

2000

Mazur A., Król J., Skorupska A. 2000. Exopysaccharide as the model molecular mediator in plant stress responce to the rhizobial infection. Konferencja "Stres w badaniach biologicznych i medycznych", Lublin, 122.

Mazur A., Lejman M., Król J., Skorupska A. 2000. The pssNOP region of Rhizobium leguminosarum bv. trifolii transcriptional activity. The Fourth European Nitrogen Fixation Conference, Sevilla, Hiszpania, str. 198.

1999

Mazur, A., Król, J., Skorupska, A. 1999. Functional analysis of pssNOP genes, involved in exopolysaccharide production in Rhizobium leguminosarum bv. trifolii TA1. I Krajowy Kongres Biotechnologii, Wroclaw, p.16.

Król, J., Skorupska, A. 1999. Homology of the genes involved in exopolysaccharide biosynthesis and modification in selected plant symbionts and pathogens. I Krajowy Kongres Biotechnologii, Wroclaw, p. 149.

1998

Kopcinska J., Lotocka B., Skorupska A., Król J. 1998. Disturbances in infection thread development in nodules induced by the exo- mutants in clover Mat. 51 Zjazdu PTBotan., Gdansk, str. 229.

Mazur A., Wielbo J., Król J., Skorupska A. 1998. Molecular characterization and symbiotic importance of prsD gene Rhizobium leguminosarum bv. trifolii TA1. J. Appl. Genet. 39.A, 171.

Król J., Mazur A., Wielbo J., Janczarek M., Skorupska A. 1998. Genetic organization of the exo region Rhizobium leguminosarum: identification of pssNOP genes. J. Appl. Genet. 39A, 173.

Janczarek M., Król J., Skorupska A. 1998. The pssB gene product of Rhizobium leguminosarum bv. trifolii is homologous to a family of inositol monophosphatases. J. Appl. Genet. 39A, 173.

Król, J., Mazur, A., Wielbo, J., Janczarek, M., Skorupska, A. 1998. Identification of the pssNOP genes of Rhizobium leguminosarum bv. trifolii. Third European Nitrogen Fixation Conference, Lunteren, Holandia, p.97.

1997

Król J., Mazur A., Sawicki R., Wielbo J., Skorupska A. 1997. ABC type protein transport system in Rhizobium leguminosarum bv. trifolii; functional relationschipo to other Gram negative bacteria. Mat. XXXIII Zjazdu PTBioch., Katowice, str. 25

1996

Janczarek M., Król J., Skorupska A. 1996. Searching for the new DNA regions controlling exopolysaccharide synthesis in Rhizobium leguminosarum bv. trifolii. Materialy XXIII Zjazdu PTM, Lódz, s.85.

Król J., Janczarek M., Skorupska A. 1996. Identification of the genes in Rhizobium leguminosarum bv. trifolii whose products are homologues to a family of ATP binding proteins. 2 nd European Nitrogen Fixation Conference & NATO Advanced Research Workshop "Biological Fixation of Nitrogen for Ecology and Sustainable Agriculture", Poznan, s.126.

1995

Król J., Skorupska A. 1995.Homology between exo region of Rhizobium leguminosarum bv. trifolii and genes controlling synthesis and export of alkaline protease in Pseudomonas aeruginosa and Erwinia chrysanthemi. Materialy XXXI Zjazdu PTBioch. W-wa, 118A.

Król J., Marek-Kozaczuk M., Skorupska A. 1995. Mutational analysis of DNA region controlling exopolysaccheride synthesis in Rhizobium leguminosarum bv. trifolii. J. Appl. Genet. vol. 36A, 34.

Marek-Kozaczuk M., Król J. Skorupska A. 1995. Tn5 mutants of Pseudomonas sp. 267 defective in siderophore synthesis. J. Appl. Genet. vol. 36A, 34.

Skorupska A, Derylo M., Król J., Marek-Kozaczuk M. 1996. Genetic and sequencing analysis of DNA region involved in synthesis of exopolysaccharide in Rhizobium leguminosarum bv. trifolii Materialy z Konferencja Sprawozdawczej Programu BiMol FNP, Gdansk ,34.

1994

Król J., Wojcieszuk A., Skorupska A. 1994. Cloning and sequencing exo region from Rhizobium leguminosarum bv. trifolii. Proc. 1-st European Nitrogen Fixation Conference, Szeged, Officina Press, p.319

…Education

…Professional Experience

…Courses

The First Baltic Sea Region Symposium and Postgraduate course “Agro-biotechnology focused on root-microbe systems”, Kaunas (Lithuania) 2 weeks, 2005 (Teacher)

Institute of Biochemistry and Biophisics Polish Academy of Science Warsaw, “DNA sequencing and sequence analyses” practical course, 1 week, 2003

Centre of Excellence in Molecular Biotechnology, Institute of Biochemistry and Biophisics Polish Academy of Science Warsaw “Advanced Theoretical Course on Signals Involved in Plant-Microbe Interactions”, Goniadz 2002

International Center for Genetic Engineering and Biotechnology (ICGEB), “Practical Course – Computer methods in molecular biology” Italy, 2 weeks, 1999

International Center for Genetic Engineering and Biotechnology (ICGEB), 10th Anniversary Symposium “Molecular Biology and Biotechnology for Development” Italy, 1 week, 1997

Institute of Bioorganic Chemistry Polish Academy of Science Poznan, “Molecular probes in molecular biology”, practical course, 1 week, 1993

…Educational Courses at University

Genetics – Mendelian Genetics, genome organization, construction of genetic and physical maps, genetic control of transcription and replication in Eucaryota and Procaryota, mutagenesis and reparation systems, restriction and modification, transformation, transduction and conjugation

Plant Biotechnology – purpose of plant biotechnology, plant genomics, use of genetic engineering methods for plant transgenesis, plant transformation methods and vector construction, reporter genes, antisense and RNAi technology

Genetic Engineering – molecular cloning and enzymes used in these methods, vectors construction and characterization, transformation and selection methods, DNA and cDNA library construction, PCR techniques, electrophoresis, Southern, Northern and Western hybridization, DNA sequencing and genome analyses, mutagenesis, DNA micro – and macroarrays

Bioinformatics – DNA sequence analyses (restriction analyses, ORF and regulatory sequences findings, contig formation, searching for repeated sequences, in silico translation), molecular data bases (GenBank, EMBL, DDJB, PIR, SwissProt), searching for information (Entrez, SRS), homology searching (Blast, FASTA, Blitz), DNA sequence submission and so on.

…Organized Courses

Institute of Microbiology and Biotechnology University of Maria Curie-Sklodowska Lublin. “Use of PCR methods in molecular diagnostic”, Practical course, 1 week, 2003

Voivodship Sanitary and Epidemiological Station in Warsaw. “Use of PCR methods in molecular diagnostic”, Practical course, 1 week, 2003

Commission of Biotechnology Polish Academy of Science Lublin Branch - Pulawy “Basics of Bioinformatics” Practical course, 1 week, 2004

Center of Biotechnology University of Maria Curie-Sklodowska Lublin “Basics of Bioinformatics” Practical course, 1 week, 2005

Center of Biotechnology University of Maria Curie-Sklodowska Lublin “Basics of Bioinformatics” Practical course, 1 week, 2005

…Honors and Awards

1st Award of Memory of Prof. Kazimierz Bassalik from Committee of Microbiology, Polish Academy of Science – 2007

2nd Award of Memory of Prof. Kazimierz Bassalik from Committee of Microbiology, Polish Academy of Science – 2004

1st Team Award of Organization Committee of The First National Biotechnology Congress, Lódz 2003
(For original papers in 2001 – 2003)

2nd Team Award of Polish Genetic Society, Poznan 2001, (For original papers in 1999 – 2001)

Award of Ministry of Education – 1999, (for the best PhD thesis)

Scholarship for Young Scientists from Foundation for Polish Science – 1999

2nd Team Award of J.M. Rector University of Maria Curie-Sklodowska –1998

Letter of Congratulations of J.M. 2nd Team Award of J.M. Rector University of Maria Curie-Sklodowska – 1996

Rector of University of Maria Curie-Sklodowska – MS with honors – 1993

…Memberships and Affiliations

Polish Genetic Society

Polish Society of Microbiologists

Microbiology Committee of The Polish Academy of Sciences (1999 – 2003)

Biotechnology Commission Polish Academy of Sciences Lublin Branch (Secretary)(2003 – present)

Faculty of Biology and Earth Sciences Board (1999 – 2005)

Scholarship for Young Scientists from Foundation for Polish Science – 1999

Institute of Microbiology and Biotechnology Board (2006 – present)

...Research specific skills

…Laboratory Skills

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